Coding

Part:BBa_K4488020:Design

Designed by: Jessica Zhang   Group: iGEM22_Sydney_Australia   (2022-10-01)


Fusion of free-use GFPb with CBDcipA (cellulose-binding domain) at the C-terminal end with a linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

One consideration was the addition of the flexible linker in order to optimise protein folding.


Source

Original GFP is derived from the jellyfish Aequorea victoria.

References